GLP-1 ELISA Kit

INTENDED USE

This high sensitive ELISA (enzyme-linked immunosorbent assay) kit is produced for the exclusively quantitative determination of glucagon-llike peptide-1 (7-36) [GLP-1 (7-36)] level in serum and EDTA-plasma sample among human, rat, mouse, goat, etc.

 

ASSAY PRINCIPLE

This ELISA is designed, developed and produced for the quantitative measurement of bioactive GLP-1 (7-36) in plasma sample. The assay utilizes the two-site “sandwich” technique with two selected GLP-1 (7-36) specific antibodies. Assay standards, controls and test samples are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 (7-36) specific antibody and a horseradish peroxidate (HRP) conjugated GLP-1 (7-36) specific antibody is added to each well. After the first incubation period, a “sandwich” immunocomplex of “Streptavidin – Biotin-Antibody – GLP-1(7-36) – HRP conjugated antibody” is formed and attached to the wall of the plate. The unbound HRP conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36) on the wall of the microtiter well is directly proportional to the amount of  GLP-1 (7-36) in the sample.

 

Active GLP-1 (7-36) ELISA

Measures GLP-1 (7-36) without cross reaction to:

– GLP-1 (9-36), (7-37), (9-37), (1-36), (1-37)

– GLP-2

– Glucagon

Assay Sensivity: 0.6 pmol/l

 

Total GLP-1 ELISA

Measures the sum value of GLP-1 (7-36) and (9-36) without cross reaction to:

– GLP-1 (7-37), (9-37), (1-36), (1-37)

– GLP-2

– Glucagon

Assay Sensivity: 1.0 pmol/l

PRODOTTI:

Active GLP-1 (7-36) ELISA Cat # EPI-KT871

Total GLP-1 ELISA Cat # EPI-KT876

GLP-1 Sample Extraction Kit Cat # EPI-KT910

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